No formal training required. Read the instructions that are available as "Links" and "Resources" at the bottom of this page or in the tool manual by the microscope.
Then contact the tool manager with any questions.
- No biosafety level-2 samples allowed (human cells, non-human primate cells, toxins or microbes that cause disease in humans)
The microscope is located in 201 Duffield. The microscope has light pathways for transmitted or reflected brightfield, and fluorescence (filters for TRITC, DAPI and FITC). The stage can accommodate devices, printed materials, and microfluidics. It is available for use with fluorescent devices, particles or cells (No cells of biosafety level-2 allowed).
The digital CCD camera (Zyla 4.2 PLUS) and image analysis software (Nikon NIS-Elements) system is available for inspection of live images as well as documentation, measurement and analysis of images. Capabilities include: time lapse acquisition, optical density analysis, autotrace feature, filtering with fast Fourier transformation, counting, measuring, classifying, color segmentation and calculating population density. Moving objects (particles, cells, etc.) can also be tracked and quantified.
- Objectives: 2.5X, 5X, 10X, 20X & 50X.
- Brightfield or Fluorescence.
- Filters: Brightfield, TRITC (Red), DAPI, Qdot WXLM (Green)
- Size measurement software (micron-scale measurements).
- Image acquisition software
- Inspection & size measurements of laser-cut devices
- Inspection & size measurements of 3D-printed devices
- Still image capture or movies of red, DAPI & green fluorescence
- Monochrome (false-color) camera capture.