For training, please contact the Tool Manager(s).
- All samples must be pre-approved by the tool manager.
- Work with samples derived from humans, non-human primates or infectious microbes must be coordinated with the tool manager.
The NanoSight (Malvern NS300) uses the technology of Nanoparticle Tracking Analysis (NTA) to characterize nanoparticles from 30 to 800 nanometers in liquid. Each particle is individually and simultaneously analyzed by direct observation and measurement of diffusion events. A laser beam is passed through the sample chamber, and the particles in suspension scatter light so they can be easily visualized by the 20X magnification microscope and camera. The camera can take 30 frames per second and captures a video file of the particles. This particle-by-particle methodology produces high-resolution results for particle size distribution and concentration
Both particle size and concentration are measured, while a fluorescence mode provides differentiation of labeled or naturally fluorescing particles. The NanoSight’s can analyze monodisperse and polydisperse samples.
- Measurement Options available:
- Size analysis (30 to 800 nm-dia.)
- Particle concentration (10^6 to 10^9 particles/mL)
- 532 nm laser available for fluorescence measurements
- Temperature range= 20 to 50 C
- Sample volume required ~1 mL
Analyze polydisperse particles: exosomes and vesicles shed from cells.
Using the device, she was able to calculate the hydrodynamic diameter of the particles to output their size distribution and concentration. Because the microvesicles are shed in a range of sizes, the NanoSight’s ability to handle polydisperse samples was essential to the research.